Intermediate
GLF

Part:BBa_K558005:Design

Designed by: Vadim Gladwill, Laura Colgin   Group: iGEM11_Nevada   (2011-09-20)

Zymomonas mobilis glucose facilitator gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 309
    Illegal NgoMIV site found at 393
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 792
    Illegal NgoMIV site found at 828
    Illegal AgeI site found at 348
    Illegal AgeI site found at 504
    Illegal AgeI site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]


codon optimized for Synechococcus elongatus PCC7942 contain C-terminal 6x histidine tag derived from Zymomonas mobilis

Source

Synthetic construct

References

Parker, C., Barnell, W. O., Snoep, J. L., Ingram, L. O. and Conway, T. (1995), Characterization of the Zymomonas mobilis glucose facilitator gene product (glf) in recombinant Escherichia coli: examination of transport mechanism, kinetics and the role of glucokinase in glucose transport. Molecular Microbiology, 15: 795–802. doi: 10.1111/j.1365-2958.1995.tb02350.x

Niederholtmeyer, Henrike, Bernd T. Wolfstader, David F. Savage, Pamela A. Silver, and Jeffrey C. Way. (2010).Engineering Cyanobacteria to Synthesize and Export Hydrophillic Products. Applied and Environmental Microbiology 76, 3462-3466. Print.